ccl 2 tm experimental models (ATCC)
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ccl 2 tm experimental models
Ccl 2 Tm Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ccl+2+tm+experimental+models/HeLa/pm40720240-277-259-257
Average 99 stars, based on 24158 article reviews
Ccl 2 Tm Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ccl+2+tm+experimental+models/HeLa/pm40720240-277-259-257
Average 99 stars, based on 24158 article reviews
ccl 2 tm experimental models - by Bioz Stars,
2026-09
99/100 stars
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Virus:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Recombinant:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Blocking Assay:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Western Blot:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells DNA Ligation:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Silver Staining:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells SYBR Green Assay:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Real-time Polymerase Chain Reaction:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells MTT Assay:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Cytotoxicity Assay:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells RNA Sequencing:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Sequencing:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells Amplification:Article Title: A trans-kingdom T6SS RNase effector targeting both prokaryotic and host cells for pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-VSVG Santa Cruz Biotechnology Cat# sc-365019; RRID: AB_10846802 Mouse monoclonal anti-RNAP BioLegend Cat# 663104; RRID: AB_2687386 Mouse monoclonal anti-GST Santa Cruz Biotechnology Cat# sc-53909; RRID: AB_783586 Mouse monoclonal anti-His Santa Cruz Biotechnology Cat# sc-8036; RRID: AB_627727 goat anti-mouse horseradish peroxidase conjugated secondary antibodies DIYIBio Cat# DY60203 Bacterial and virus strains Bacterial strains This study Table S2 Chemicals, peptides, and recombinant proteins ApexHF HS DNA Polymerase FS Master Mix Accurate Biotechnology Cat# AG12202 QuickBlock TM Blocking Buffer for Western Blot Beyotime Biotechnology Cat# P0252 QuickBlock TM Primary Antibody Dilution Buffer for Western Blot Beyotime Biotechnology Cat# P0256 IPTG (Isopropyl β-DThiogalactopyranoside) Solarbio Cat# I8070 ONPG (o-Nitrophenyl-β-DGalactopyranoside) Solarbio Cat# O8040 GST⋅Bind TM Resin Millipore Sigma Cat# 70541 Ni-NTA His⋅Bind® Resin Millipore Sigma Cat# 70666 MagneGST TM Glutathione Particles Promega Cat# V8612 Alexa Fluor TM 488 C 5 Maleimide Thermo Fisher Scientific Cat# A10254 Fetal Bovine Serum, FBS Beyotime Biotechnology Cat# C0234 Critical commercial assays DNA Ligation Kit Accurate Biotechnology Cat# AG11801 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat# DP430 Pierce TM Silver Stain Kit Thermo Fisher Scientific Cat# 24612 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biotechnology Cat# AG11701 LiveBLAzer TM FRET-B/G Loading Kit with CCF2-AM Invitrogen Cat# K1032 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat# C0009S Annexin V-FITC Apoptosis Detection Kit Beyotime Biotechnology Cat# C1062M Deposited data E. coli RNA-seq data This study NCBI Sequence Read Archive: PRJNA1130366 16S rRNA amplicon sequencing data This study NCBI Sequence Read Archive: PRJNA1141948 Experimental models: Cell lines Human: HeLa cells |